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Animal Health and Diagnostics

 

See below for project activity in this area

Project title: Priority Animal Health Testing – Rapid on-Farm Electronic Diagnostics

Researchers: Simon Egan, Baljit Singh, Brian Seddon

BovAlert video http://youtu.be/IdJcgIjEMHs

Objective: The veterinary point-of-need diagnostics field, where decentralised tests are performed on animals for the immediate detection of pathogens is virtually non-existent. This “animal point of care diagnostics” market has major market niches for point-of-need diagnostics in the control and monitoring of farm animals, but also for small animal testing at veterinary surgeries and indeed as consumer products with customer-based testing, where animal owners self-test their animals for common ailments such as pathogen infection and allergy. This project has progressed an electrochemical ELISA approach for measurement of the parasite liverfluke in bovine milk/serum samples (see http://www.micra.ie).

Collaborators: Grace Mulcahy and Mary Sekiya, School of Veterinary Medicine UCD

Funding: Enterprise Ireland Commercialisation fund.

Outputs: Bibliographic data: WO2014076207 (A1) ― 2014-05-22, MICRO-REAGENT HANDLER AND CARTRIDGE ASSEMBLY, SEDDON BRIAN [IE]; O'REILLY RONAN [IE]; DEMPSEY EITHNE [IE]; HAYES JAMES [IE] +
 

 

 

 

 

 

 

 

 

 

 

 

Project title: Evaluation of a pico-molar sensitive nano-gold immuno-electrochemical technique (ALISA)


Postgraduate researchers: Jessica Kelch


Objective: Synthesis and application of immunochemically modified gold nano-materials will advance knowledge on their colloidal stabilisation and fundamental redox properties. The project will demonstrate synthesis routes for novel linker thione molecules (ligands) and their self-assembly, co-ordination complex reactivity, to nano-Au leading to detection systems for electronic progesterone immunoassay systems suitable for on-farm pregnancy testing.

Collaborators: Mark Crowe, UCD School of Veterinary Medicine – Hormone Assay Laboratory, and industrial partner Mintek, South Africa (a mineral research company)].

Funding: Science Foundation Ireland Investigator Award

Outputs: Oral and poster presentations at national and international conferences

 

 

 

 

 

AFM images of Au nanoparticles


 

 

 

 

 

 

 

 

Project Title: Demonstration of a capillary-flow Point of Care (PoC) device “Immuno-CAP” for the measurement of progesterone in bovine milk.

Postgraduate researcher: Aoife Delaney

Objective: Design and fabrication of a diagnostic point of care progesterone test device realises a micro-fluidic component which integrates capillary immunochromatography principles with gold electrolysis. The assay scheme proposed involves competition between free progesterone (in the sample) and gold-labelled progesterone conjugate with the anti-progesterone reagent immobilised within a micro-capillary channel. The quantity of conjugate surviving the capillary transportation process is detected as it arrives at a printed electronic sensor.

Collaborators: Mark Crowe, UCD School of Veterinary Medicine – Hormone Assay Laboratory, and industrial partner Mintek, South Africa (a mineral research company)].

Funding: Science Foundation Ireland Investigator Award

Outputs: Oral and poster presentations at national and international events

 

 

 

 

 

 

 

 

 

 

 

 

Project Title: The development of early non-invasive and reliable molecular biomarkers of pregnancy in dairy cattle.

Researcher: To be recruited

Objective: The proposed activity builds on the above project involving an electronic portable device for the point-of-care (PoC) monitoring of progesterone. Here we propose addition of selected biomarkers (PAG, IgG glycan) onto the panel with the view to facilitate multiplexed detection capability in blood/milk. In order to achieve this, current ELISA formats (where available) will be adapted onto a novel platform, building upon know-how in electrochemical immunoassay formats coupled with the exploitation of suitable nanoparticle labels such as colloidal gold, quantum dots.


Collaborators: Mark Crowe, UCD School of Veterinary Medicine , Michael Diskin Teagasc, Pauline Rudd NIBRT Dublin.

Funding: Science Foundation Ireland Investigator Award

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