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Cytotoxicity Microsystems

 

Project title: Toxor: Design and Application of an Electrochemical Toxicity Biosensor for Environmental Monitoring

Postgraduate researcher: Tony O’Hara

Objective: A whole cell electrochemical biosensor has been developed which monitors changes in cellular enzyme acid phosphatase (AP) in eukaryotic cells on exposure to toxic chemicals. Redox molecule generation by cells is measured electrochemically and IC50 for priority pollutants and nanomaterials (quantum dots) correlated vs. standard MTT assays.  TOXOR enables evaluation of a novel bioelectronic device for the monitoring of cellular enzyme activity to assess "chemical stress" on living cells. The cell-electrode interface in development at ITT Dublin offers superior information acquisition opportunities for molecular toxicity screening and toxic-agent detection.


Collaborators: Siobhan McClean (CMHI, ITT Dublin), Eric Moore, Tyndall National Institute Cork


Funding: Irish Research Council (IRC)

Outputs:

Evaluation of quantum dot nanotoxicity to human lung cells using TOXOR electronic enzyme assay methodology, Tony O’Hara, Brian Seddon, Siobhán McClean, Baljit Singh, Dimitri Scholz and Eithne Dempsey, submitted ACS Sensors October 2015

Tony O’Hara, Brian Seddon, Siobhán McClean and Eithne Dempsey*, ‘TOXOR: Design and Application of an Electrochemical Toxicity Biosensor for Environmental Monitoring’, Electroanalysis, Volume 27, Issue 1, pages 58–66, January 2015.
 

 

 A sensor locus in the bio-electrode array

 

“TOXOR" - links electronics and cellular chemistry. The TOXOR bio-electrode is a single measurement cell-based printed sensor for the rapid assessment of toxic environments.

 

 

                                          

 

 

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